HiMedia M384-100G Zobell Marine Agar 2216 100g Laboratory Grade

Description

Product Overview

The HiMedia M384-100G Zobell Marine Agar 2216 is formulated to support the growth of a broad spectrum of marine heterotrophic bacteria. Manufactured in India by HiMedia Laboratories, this medium combines a balanced blend of peptone, yeast extract, and marine salts to mimic natural seawater conditions, ensuring optimal nutrient availability. The 100 g package provides ample material for multiple experiments while maintaining consistent performance across batches. Its sterile, powder‑free composition reduces contamination risk, and the product meets stringent quality standards required for research, quality control, and industrial applications. The medium is compatible with standard incubation temperatures ranging from 20 °C to 30 °C, making it versatile for diverse marine microbiology protocols.

Usage

Designed for laboratory and field researchers, this agar is ideal for isolating marine bacteria from seawater, sediment, and marine organism samples. It supports routine plate counts, enrichment cultures, and selective isolation of specific marine strains. The medium is also suitable for educational settings, allowing students to observe colony morphology and conduct biochemical tests. Its reliable performance under varied incubation conditions makes it a preferred choice for environmental monitoring, aquaculture health assessments, and biotechnological research focused on marine enzymes, bioactive compounds, and bioremediation studies.

Why Choose Us

HiMedia leverages over three decades of experience in microbiological media production, ensuring each batch of Zobell Marine Agar meets rigorous purity and performance criteria. The product is manufactured under ISO‑9001 certified processes, with comprehensive batch testing for sterility, pH stability, and nutrient consistency. Customers benefit from a reliable supply chain, transparent sourcing of raw materials, and a commitment to continuous improvement. By choosing HiMedia, laboratories gain confidence in reproducible results, reduced experimental variability, and access to responsive technical support that can assist with protocol optimization and troubleshooting.

Performance

Extensive validation studies demonstrate that HiMedia Zobell Marine Agar consistently yields high colony‑forming unit (CFU) counts comparable to reference media from leading suppliers. The medium supports rapid colony development, typically visible within 24‑48 hours, and maintains colony integrity for extended observation periods. Its formulation promotes uniform diffusion of nutrients, minimizing gradient effects that can affect growth patterns. Researchers have reported enhanced recovery of fastidious marine strains, facilitating downstream applications such as genome sequencing, metabolite profiling, and strain preservation.

Quality Assurance

Each production lot undergoes a multi‑stage quality control protocol, including raw material verification, in‑process sterility checks, and final product testing for pH (7.2 ± 0.2) and moisture content (<5%). Certificates of analysis (CoA) are provided upon request, detailing microbial limits, endotoxin levels, and confirmatory test results. The medium is stored in moisture‑resistant, sealed containers to preserve its integrity during transport and handling.

Packaging & Shipping

The 100 g package is sealed in a tamper‑evident, moisture‑proof pouch that protects the agar from humidity and contamination. Shipping is performed via standard courier services with temperature‑controlled options available for bulk orders. Packaging dimensions of 4.84 × 2.72 × 2.72 inches and a weight of 6 ounces facilitate easy handling and storage in laboratory cabinets or refrigerators.

Storage & Shelf Life

For optimal performance, store the agar at 2 °C – 8 °C in a dry environment. The product retains full potency for up to 24 months from the date of manufacture when stored under recommended conditions. Once opened, the medium should be used within 30 days to prevent moisture absorption and microbial contamination.

Customer Support

HiMedia offers dedicated technical assistance through email, phone, and online chat. Our experts can provide guidance on media preparation, inoculation techniques, and troubleshooting unexpected growth patterns. Additionally, we supply detailed protocol sheets and application notes to help users integrate the agar into existing workflows efficiently.

Regulatory Compliance

The product complies with international standards for laboratory reagents, including ISO 13485 for medical device‑related applications and OECD guidelines for environmental testing. It is free from animal‑derived components, making it suitable for laboratories adhering to ethical sourcing policies.

Application Examples

Researchers have employed HiMedia Zobell Marine Agar to isolate Vibrio spp. from coastal water samples, assess the impact of pollutants on marine microbial communities, and screen for novel antimicrobial compounds produced by marine actinomycetes. In aquaculture, the medium assists in monitoring pathogenic bacteria levels in hatchery water, supporting proactive disease management strategies.

Technical Specifications

Ingredient composition (per liter): Peptone 5 g, Yeast extract 1 g, Ferric citrate 0.01 g, Sodium chloride 19.45 g, Magnesium chloride 8.8 g, Calcium chloride 0.8 g, Sodium sulfate 3.24 g, Potassium bromide 0.8 g, Sodium nitrate 0.5 g, Sodium silicate 0.2 g, Sodium bicarbonate 0.1 g, Agar 15 g. pH 7.2 ± 0.2 at 25 °C. Moisture content <5 %. Sterile, powder‑free, ready‑to‑use after autoclaving at 121 °C for 15 minutes.

Key Features

• Optimized for robust growth of diverse marine heterotrophic bacteria
• Consistent batch‑to‑batch performance ensures reproducible experimental results
• Moisture‑proof packaging preserves sterility and extends shelf life up to 24 months
• ISO‑9001 certified manufacturing guarantees high purity and low endotoxin levels
• Responsive technical support and detailed application notes for seamless integration

FAQ

What is the recommended preparation method for Zobell Marine Agar?

Dissolve the entire 100 g powder in 1 L of distilled water, adjust the pH to 7.2 if necessary, and autoclave at 121 °C for 15 minutes. Allow the medium to cool to 45‑50 °C before pouring plates.

Can this agar be used for selective isolation of specific marine bacteria?

Yes, the medium can be supplemented with antibiotics or selective agents to target particular genera such as Vibrio or Pseudomonas, while still providing the essential marine salts required for growth.

Is the product suitable for educational laboratory courses?

Absolutely. Its clear formulation, reliable performance, and safety profile make it an excellent choice for teaching microbiology techniques, colony morphology observation, and basic biochemical testing in classroom settings.

How does HiMedia ensure the sterility of the agar?

All raw materials undergo microbial limit testing, and the final product is sealed in sterile, moisture‑resistant pouches. Batch sterility is verified by incubating a sample under non‑selective conditions to confirm the absence of contaminating organisms.

What should I do if I notice unexpected growth patterns?

Contact HiMedia technical support with details of your protocol, incubation conditions, and any deviations observed. Our experts can help troubleshoot potential issues such as pH shifts, contamination, or nutrient imbalances.